Database : HANSEN Search on : PRIMERS DO DNA [Subject descriptor] References found : 22 [refine] Displaying: 1 .. 20   in format [Detailed]

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Id: 17283 Author: Osaki, Mitsuhiko; Adachi, Hironobu; Gomyo, Yoshihito; Yoshida, Haruhiko; Ito, Hisao Title: Detection of mycobacterial DNA in formalin-fixed, paraffin-embedded tissue specimens by duplex polymerase chain reaction: application to histopathologic diagnosis ..- Source: s.l; s.n; 1997. 6 p. ilus, tab. Abstract: Granuloma is a chronic inflammatory process associated with noninfectious agents or infectious diseases such as tuberculosis. Determination of the causative agent might be occasionally difficult in histopathologic sections. In this study, we examined 60 specimens of granuloma or inflammatory lesions that were originally diagnosed as 51 cases of granulomatous inflammation, 6 of leprosy, and 3 of atypical mycobacteriosis. The diagnoses in the last two categories were made both histologically and clinically. All of the sections and DNA were prepared from formalin-fixed, paraffin-embedded blocks. Histopathologic and immunohistochemical findings were compared with the results of duplex polymerase chain reaction (PCR) using two primers to amplify mycobacterial-common 383-base pair (bp) DNA and Mycobacterium tuberculosis-complex-specific 240-bp DNA. Six samples of leprosy and three of atypical mycobacteriosis showed the 383-bp but not the 240-bp band. Among the 51 specimens of granulomatous inflammations, nine showed no band of even the beta-globin, the cases being excluded from this analysis. The 42 specimens of granulomatous inflammation were subdivided into three categories by PCR: (1) 383- and 240-bp positive; (2) 383-bp positive and 240-bp negative; and (3) both negative. Category 1 included 32 specimens (76.2%), being considered as tuberculosis. One specimen was classified into Category 2, indicating possible atypical mycobacterium. Category 3 included nine specimens, composed of five of sarcoidosis and four other agent-induced granulomas, when compared with histologic and clinical findings. These findings indicate that the PCR assay using DNA extracted from paraffin-embedded materials provides useful information to differentiate tuberculosis from other type of granulomas. (AU). Descriptors: Primers do DNA DNA Bacteriano/*AN Diagnóstico Diferencial Fixadores Formaldeído Granuloma/*DI Micobactérias Atípicas/GE Mycobacterium/*GE Mycobacterium leprae/GE Mycobacterium tuberculosis/GE Inclusão em Parafina Reação em Cadeia da Polimerase Tuberculose/*DI Limits: Humano Location: BR191.1; 09184/s

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Id: 17276 Author: Qinxue, Wu; Xinyu, Li; Wei, Hou; Tao, Li; Yaoping, Ying; Jinping, Zhang; Xiuling, Cai; Ganyun, Ye Title: A study on PCR for detecting infection with M. leprae ..- Source: s.l; s.n; 1999. 5 p. tab. Abstract: OBJECTIVE: So far, it has not been established a satisfactory method for early diagnosis and studying on epidemiology for leprosy, we want to develop a molecular biological method for solving this point. MATERIALS AND METHODS: Based on the M. leprae gene coding groEL, 65 kD and 16S rRNA, three polymerase chain reactions were developed by using Plikaytis', Woods' and Pattyn's procedures. It was optimized that the experimental parameters for each PCR, and a comparative study on practivity among three PCRs was also conducted for practical purpose. RESULTS AND CONCLUSION: For detecting infection with M. leprae, all of PCRs established by us were highly sensitive and specific, but for practical purpose, the Woods' PCR optimized by us ought to be chosen firstly. (AU). Descriptors: Estudo Comparativo Primers do DNA DNA Bacteriano/GE Proteína GroEL/GE Hanseníase/*DI/GE/MI Mycobacterium leprae/*GE Mycobacterium tuberculosis/GE Reação em Cadeia da Polimerase/*MT RNA Ribossômico 16S/GE Sensibilidade e Especificidade Limits: Humano Location: BR191.1; 09175/s

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Id: 13741 Author: Fiallo, Paolo; Clapasson, Andrea; Favre, Anna; Pesce, Carlo Title: Overexpression of vascular endothelial growth factor and its endothelial cell receptor KDR in type I leprosy reaction ..- Source: s.l; s.n; Feb. 2002. 6 p. ilus, tab. Abstract: The sites of expression of vascular endothelial growth factor (VEGF) and of KDR, its endothelial cell receptor, were investigated in leprosy reaction Type 1, or reversal reaction (RR), by immunohistochemistry and in situ hybridization. In comparison with nonreactional leprosy, overexpression of both VEGF and KDR was seen in granuloma cells, especially epithelioid and foreign body-type giant cells, the epithelium and the vascular endothelium of RR specimens. In granuloma cells, hybridization for VEGF was stronger than immunostaining, a finding that may reflect the rapid turnover of VEGF in an immunologically dynamic situation such as RR. In the epidermis, double immunohistochemistry revealed VEGF overexpression in CDla-positive dendritic cells. The VEGF may not only be relevant for hyperpermeability and mononuclear cell differentiation (the key morphologic features in the acute, clinically evident phase of RR), but it could also be implicated in RR onset, when dendritic cells are activated in response to antigen stimulation. (AU). Descriptors: PRIMERS DO DNA FATORES DE CRESCIMENTO ENDOTELIAL/metab HANSENIASE DIMORFA/metab HANSENIASE DIMORFA/patol RECEPTORES DOS FATORES DE CRESCIMENTO/metab RECEPTORES MITOGÊNICOS/metab LINFOCINAS/metab RECEPTORES PROTEINA TIROSINA QUINASES/metab IMUNOHISTOQUIMICA HIBRIDIZACAO IN SITU - INCLUSAO EM PARAFINA Limits: HUMANO SUPPORT, NON-U.S. GOV'T RECEPTORES DE FATOR DE CRESCIMENTO DO ENDOTELIO VASCULAR Electronic Medium: http://www.ilsl.br Location: BR191.1; 09033/s

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Id: 13719 Author: Kang, T. -J; Kim, S. -K; Lee, S. -B; Chae, G. -T; Kim, J. -P Title: Comparison of two different PCR amplification products (the 18-kDa protein gene vs. RLEP repetitive sequence) in the diagnosis of Mycobacterium leprae ..- Source: s.l; s.n; 2003. 5 p. tab. Abstract: To determine the best molecular method for diagnosing leprosy, two sets of Mycobacterium leprae-specific primers were compared. Fresh biopsies and slit skin smear samples were obtained from 67 leprosy patients and examined by touchdown (TD) PCR using primers amplifying either a 129-bp fragment of the RLEP repetitive sequence or a 360-bp fragment of the 18-kDa protein gene of M. leprae. Seventeen of 30 (56.7 per cent) biopsy specimens and four of 37 (10.8 per cent) slit skin smear specimens were positive using the primer for the 18-kDa protein gene, whereas 24 of 30 (80 per cent) biopsy and 27 of 37 (73 per cent) slit skin smear samples showed detectable PCR products in the RLEP repetitive sequence. Twenty-one of 31 cases (67.7 per cent) with a bacterial index of zero were PCR positive for the primer RLEP repetitive sequence. These results demonstrate that detection of M. leprae using PCR with primers to a RLEP sequence is more sensitive and specific than PCR with the 18-kDa protein gene primers and also slit smears with acid fast staining. PCR of RLEP repetitive sequences is therefore a useful means of detecting M. leprae DNA even when it is present at very low levels. (AU). Descriptors: ANTICORPOS ANTIBACTERIAS/sangue PROTEINAS DE BACTERIAS/genet HANSENIASE/diag HANSENIASE/microbiol MYCOBACTERIUM LEPRAE/genet MYCOBACTERIUM LEPRAE/isol REACAO EM CADEIA DA POLIMERASE/métodos PELE/microbiol - SEQUÊNCIAS REPETITIVAS DO ACIDO NUCLEICO  PRIMERS DO DNA Limits: ESTUDO COMPARATIVO HUMANO Electronic Medium: http://www.ilsl.br Location: BR191.1; 09117/s

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Id: 13639 Author: Kang, T. -J; Kim, S. -K; Lee, S. -B; Chae, G. -T; Kim, J. -P Title: Comparison of two different PCR amplification products (the 18-kDa protein gene vs. RLEP repetitive sequence) in the diagnosis of Mycobacterium leprae ..- Source: s.l; s.n; 2003. 5 p. ilus, tab. Abstract: To determine the best molecular method for diagnosing leprosy, two sets of Mycobacterium leprae-specific primers were compared. Fresh biopsies and slit skin smear samples were obtained from 67 leprosy patients and examined by touchdown (TD) PCR using primers amplifying either a 129-bp fragment of the RLEP repetitive sequence or a 360-bp fragment of the 18-kDa protein gene of M. leprae. Seventeen of 30 (56.7 per cent) biopsy specimens and four of 37 (10.8 per cent) slit skin smear specimens were positive using the primer for the 18-kDa protein gene, whereas 24 of 30 (80 per cent) biopsy and 27 of 37 (73 per cent) slit skin smear samples showed detectable PCR products in the RLEP repetitive sequence. Twenty-one of 31 cases (67.7 per cent) with a bacterial index of zero were PCR positive for the primer RLEP repetitive sequence. These results demonstrate that detection of M. leprae using PCR with primers to a RLEP sequence is more sensitive and specific than PCR with the 18-kDa protein gene primers and also slit smears with acid fast staining. PCR of RLEP repetitive sequences is therefore a useful means of detecting M. leprae DNA even when it is present at very low levels. (AU). Descriptors: ANTICORPOS ANTIBACTERIAS/sangue PROTEINAS DE BACTERIAS/genet PRIMERS DO DNA HANSENIASE/diag HANSENIASE/microbiol MYCOBACTERIUM LEPRAE/genet MYCOBACTERIUM LEPRAE/isol REACAO EM CADEIA DA POLIMERASE/métodos SEQUÊNCIAS REPETITIVAS DO ACIDO NUCLEICO PELE/microbiol Limits: ESTUDO COMPARATIVO HUMANO Electronic Medium: http://www.ilsl.br Location: BR191.1; 09054/s

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Id: 12309 Author: Kang, Tae-Jin; You, Ji-Chang; Chae, Gue-Tae Title: Identification of catalase-like activity from mucobacterium leprae and the relationship between catalase and isonicotinic acid hydrazide (INH) ..- Source: s.l; s.n; 2001. 7 p. ilus, tab, graf. Descriptors: SULFATO DE AMONIO ANTITUBERCULOSOS SEQUENCIA DE BASES BENZIDINAS CATALASE CATALASE PRIMERS DO DNA DNA COMPLEMENTAR ELETROFORESE EM GEL DE AGAR PEROXIDO DE HIDROGENIO ISONIAZIDA HANSENIASE HANSENIASE MACROFAGOS PERITONEAIS CAMUNDONGOS CAMUNDONGOS ENDOGAMICOS BALB C MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE - PEROXIDASE  PEROXIDASES  PEROXIDASES  REAÇAO EM CADEIA POR POLIMERASE  DNA POLIMERASE DIRIGIDO POR RNA  CONTAGEM DE CINTILAÇAO  HOMOLOGIA DE SEQUENCIA DO ACIDO NUCLÉICO  ESPECTROFOTOMETRIA Location: BR191.1; 08638/s

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Id: 12292 Author: Ohara, Naoya; Matsuoka, Masanori; Nomaguchi, Hiroko; Naito, Mariko; Yamada, Takeshi Title: Protective responses against experimental mycobacterium leprae infection in mice induced by recombinant Bacillus Calmette-Guérin over-producing three putative protective antigen candidates ..- Source: s.l; s.n; Feb. 2001. 5 p. ilus, graf. Descriptors: ANTIGENOS DE BACTÉRIAS VACINA BCG VACINA BCG VACINA BCG SEQUENCIA DE BASES PRIMERS DO DNA HANSENIASE HANSENIASE CAMUNDONGOS CAMUNDONGOS ENDOGAMICOS BALB C CAMUNDONGOS ENDOGAMICOS C57BL MYCOBACTERIUM BOVIS MYCOBACTERIUM BOVIS Limits: ANIMAL Location: BR191.1; 08623/s

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Id: 12238 Author: Maeda, Shinji; Matsuoka, Masanori; Nakata, Noboru; Kai, Masanori; Maeda, Yumi; Hashimoto, Ken; Kimura, Hiroaki; Kobayashi, Kazuo; Kashiwabara, Yoshiko Title: Multidrug resistant mycobacterium leprae from patients with leprosy ..- Source: s.l; s.n; Dec. 2001. 5 p. tab, graf. Descriptors: AGENTES ANTIINFECCIOSOS DE FLUOROQUINOLONA AGENTES ANTIINFECCIOSOS DE FLUOROQUINOLONA ANTIBIOTICOS ANTITUBERCULOSE ANTIBIOTICOS ANTITUBERCULOSE SEQUENCIA DE BASES PRIMERS DO DNA DNA BACTERIANO DNA BACTERIANO RESISTENCIA MICROBIANA A DROGAS RESISTENCIA A MULTIPLAS DROGAS GENES BACTERIANOS GENES BACTERIANOS LEPROSTATICOS LEPROSTATICOS HANSENIASE HANSENIASE CAMUNDONGOS DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE OFLOXACINA OFLOXACINA RIFAMPINA RIFAMPINA REACAO EM CADEIA POR POLIMERASE VIA TRANSCRIPTASE REVERSA Limits: ANIMAL Location: BR191.1; 08640/s

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Id: 11947 Author: Fiallo, Paolo; Clapassom, Andrea; Favre, Anna; Pesce, Carlo Title: Overexpression of vascular endothelial growth factor and its endothelial cell receptor KDR in type 1 leprosy reaction ..- Source: s.l; s.n; 2002. 6 p. ilus, tab. Descriptors: HANSENIASE DIMORFA HANSENIASE DIMORFA PRIMERS DO DNA FATORES DE CRESCIMENTO ENDOTELIAL IMUNOHISTOQUIMICA HIBRIDIZAÇAO IN SITU LINFOCINAS INCLUSAO EM PARAFINA RECEPTORES DOS FATORES DE CRESCIMENTO RECEPTORES MITOGENICOS Location: BR191.1; 08598/s

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Id: 11686 Author: Ohara, Naoya; Matsuoka, Masanori; Nomaguchi, Hiroko; Naito, Mariko; Yamada, Takeshi Title: Protective responses against experimental Mycobacterium leprae infection in mice induced by recombinant Bacillus Calmette-Guérin over-producing three putative protective antigen candidates ..- Source: s.l; s.n; 2001. 5 p. ilus, graf. Descriptors: MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM BOVIS MYCOBACTERIUM BOVIS HANSENIASE HANSENIASE ANTIGENOS DE BACTÉRIAS VACINA BCG VACINA BCG VACINA BCG SEQUENCIA DE BASES PRIMERS DO DNA CAMUNDONGOS ENDOGAMICOS BALB C CAMUNDONGOS ENDOGAMICOS C57BL PLASMIDEOS BAÇO Limits: ANIMAL MASCULINO CAMUNDONGOS Location: BR191.1; 08478/s

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Id: 11594 Author: Martin, E; Roche, P. W; Triccas, J. A; Britton, W. J Title: DNA encoding a single mycobacterial antigen protects against leprosy infection ..- Source: s.l; s.n; 2001. 6 p. tab. Descriptors: ANTIGENOS DE BACTÉRIAS VACINA BCG VACINAS BACTERIANAS VACINAS BACTERIANAS VACINAS BACTERIANAS SEQUENCIA DE BASES PRIMERS DO DNA DNA BACTERIANO DNA BACTERIANO INTERFERON TIPO II HANSENIASE HANSENIASE HANSENIASE CAMUNDONGOS MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE LINFOCITOS T VACINAS DE DNA/GE/IM/*PD Limits: ANIMAL Location: BR191.1; 08417/s

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Id: 11495 Author: Kang, Tae-Jin; You, Ji-Chang; Chae, Gue-Tae Title: Identification of catalase like activity from mycobacterium leprae and the relationship between catalase and isonicotinic acid hydrazide (INH) ..- Source: s.l; s.n; 2001. 7 p. ilus, tab, graf. Descriptors: HANSENIASE HANSENIASE ANTITUBERCULOSOS BENZIDINAS CATALASE CATALASE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE PRIMERS DO DNA DNA COMPLEMENTAR ELETROFORESE EM GEL DE AGAR PEROXIDO DE HIDROGENIO ISONIAZIDA MACROFAGOS PERITONEAIS PEROXIDASE PEROXIDASE REAÇAO EM CADEIA POR POLIMERASE CONTAGEM DE CINTILAÇAO HOMOLOGIA DE SEQUENCIA DO ACIDO NUCLÉICO Location: BR191.1; 08385/s

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Id: 11299 Author: Moraes, M. O; Sarno, E. N; Almeida, A. S; Saraiva, B. C. C; Nery, J. A. C; Martins, R. C. L; Sampaio, E. P Title: Cytokine mRNA expression in leprosy: a possible role for interferon-gamma and interleukin-12 in reactions (RR and ENL) ..- Source: s.l; s.n; 1999. 9 p. ilus, tab, graf. Descriptors: SEQUENCIA DE AMINOACIDOS SEQUENCIA DE BASES ESTUDOS DE CASOS E CONTROLES CITOCINAS PRIMERS DO DNA EXPRESSAO GENICA FATOR ESTIMULADOR DE COLONIAS DE GRANULOCITOS-MACROFAGOS INTERFERON TIPO II INTERLEUCINA-12 HANSENIASE HANSENIASE GLICOPROTEINAS DE MEMBRANAS REAÇAO EM CADEIA POR POLIMERASE RNA MENSAGEIRO RNA MENSAGEIRO RNA MENSAGEIRO Location: BR191.1; 07238/s

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Id: 11157 Author: Donoghue, H. D; Holton, J; Spigelman, M Title: PCR primers that can detect low levels of mycobacterium leprae DNA ..- Source: s.l; s.n; 2001. 6 p. ilus, tab. Descriptors: HANSENIASE HANSENIASE HANSENIASE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE PRIMERS DO DNA DNA DNA HISTORIA DA MEDICINA MEDIEVAL PALEOPATOLOGIA POLONIA REAÇAO EM CADEIA POR POLIMERASE SENSIBILIDADE E ESPECIFICIDADE Location: BR191.1; 07307/s

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Id: 11066 Author: Rajalingam, R; Mehra, N. K; Singal, D. P Title: Polymorphism in heat-shock protein 70-1(HSP70-1) gene promoter region and susceptibility to tuberculoid leprosy and pulmonary tuberculosis in Asia Indians ..- Source: s.l; s.n; jul. 2000. 5 p. tab. Descriptors: HANSENIASE TUBERCULOIDE HANSENIASE TUBERCULOIDE PRIMERS DO DNA ANTIGENOS HLA PROTEINAS 70 DE CHOQUE TÉRMICO POLIMORFISMO (GENÉTICA) REGIOES PROMOTORAS (GENÉTICA) TUBERCULOSE PULMONAR TUBERCULOSE PULMONAR Location: BR191.1; 07362/s

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Id: 10299 Author: Roy, S; Frodsham, A; Saha, B; Hazra, K. S; Maccie-Taylor, C. G. N; Hill, A. V. S Title: Association of vitamin D receptor genotype with leprosy type ..- Source: s.l; s.n; 1999. 5 p. tab. Descriptors: ALELOS SEQUENCIA DE BASES PROTEINAS PRIMITIVAS DE ADENOVIRUS PROTEINAS DE TRANSPORTE ESTUDOS DE CASOS E CONTROLES PRIMERS DO DNA FREQUENCIA DOS GENES GENOTIPO IMUNOGENÉTICA INDIA HANSENIASE HANSENIASE HANSENIASE LEPROMATOSA HANSENIASE LEPROMATOSA HANSENIASE TUBERCULOIDE HANSENIASE TUBERCULOIDE PROTEINAS DA MEMBRANA POLIMORFISMO (GENÉTICA) RECEPTORES DE CALCITRIOL RECEPTORES DE CALCITRIOL DELEÇAO DE SEQUENCIA VARIAÇAO (GENÉTICA) Location: BR191.1; 07402/s

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Id: 10245 Author: Sieling, Peter A; Wang, Xiao-Hong; Gately, Maurice K; Oliveros, Jennifer L; McHugh, Teresa; Barnes, Peter F; Wolf, Stanley F; Golkar, Linda; Yamamura, Masahiro; Yogi, Yasuko; Uyemura, Koichi; Rea, Thomas H; Modlin, Robert L Title: IL-12 regulates T helper type 1 cytokine responses in human infectious disease ..- Source: s.l; s.n; 1994. 9 p. ilus, graf. Descriptors: SEQUENCIA DE BASES LINFOCITOS T CD8-POSITIVOS PRIMERS DO DNA EXPRESSAO GENICA INTERFERON TIPO II INTERLEUCINA-10 INTERLEUCINA-12 INTERLEUCINA-4 INTERLEUCINA-4 HANSENIASE TRANSFORMAÇAO LINFOCITICA DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM LEPRAE RNA MENSAGEIRO SUB-POPULAÇOES DE LINFOCITOS T CÉLULAS TH1 Location: BR191.1; 06955/s

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Id: 10214 Author: Qinxue, Wu; Qingying, Kong; Xinyu, Li; Wanhui, Wei; Ganyun, Ye Title: Integration of traditional and modern methods in the identification of AFB cultures isolated from clinical specimens of petients with skin diseases ..- Source: s.l; s.n; 1994. 5 p. ilus, tab. Descriptors: SEQUENCIA DE BASES PRIMERS DO DNA ELISA HANSENIASE DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM LEPRAE MYCOBACTERIUM TUBERCULOSIS REAÇAO EM CADEIA POR POLIMERASE SARCOIDOSE DERMATOPATIAS Location: BR191.1; 06915/s

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Id: 10171 Author: Richter, Elvira; Duchrow, Michael; Schluter, Carsten; Hahn, Margrit; Flad, Hans-D; Gerdes, Johannes Title: Detection of Mycobacterium leprae by three-primer PCR ..- Source: s.l; s.n; 1994. 3 p. ilus. Descriptors: SEQUENCIA DE BASES PRIMERS DO DNA ESTUDOS DE AVALIAÇAO HANSENIASE DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE REAÇAO EM CADEIA POR POLIMERASE RNA BACTERIANO RNA RIBOSSOMICO 16S ESPECIFICIDADE DE ESPÉCIES Location: BR191.1; 07084/s

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Id: 10166 Author: Struyk, Linda; Hawes, Gail E; Haanen, John B. A. G; Vries, Rene R. P. de; Van den Elsen, Peter J Title: Clonal dominance and selection for similar complementarity determining region 3 motifs among T lymphocytes responding to the HLA-DR3-associated Mycobacterium leprae shock protein 65-kd peptide 3-13 ..- Source: s.l; s.n; 1995. 8 p. ilus, tab. Descriptors: HANSENIASE TUBERCULOIDE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE LINFOCITOS T SEQUENCIA DE AMINOACIDOS PROTEINAS DE BACTÉRIAS SEQUENCIA DE BASES LINHAGEM CELULAR CHAPERONINAS CHAPERONINAS PRIMERS DO DNA ANTIGENO HLA-DR3 REGIAO VARIAVEL DE IMUNOGLOBULINA DADOS DE SEQUENCIA MOLECULAR FRAGMENTOS DE PEPTIDIOS FRAGMENTOS DE PEPTIDIOS RECEPTORES PARA ANTIGENOS DE CÉLULAS T ALFA-BETA Limits: HUMANO Location: BR191.1; 07079/s

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